ABSTRACT
Objective To analyze the resistance genes of multidrug-resistant Pseudomonas aeruginosa by high throughput sequencing and bioinformatics.Methods Multiple drug-resistant strains were screened from 246 strains of Pseudomonas aeruginosa.The genome and plasmid DNA of target bacteria were extracted by al-kaline lysis and sequenced by high throughput.The resistance genes were analyzed by bioinformatics.Results 102 strains of multidrug resistant strains were detected,21 strains of extensively drug resistant strains of poly-myxin B sensitive.102 strains of multidrug resistant Pseudomonas aeruginosa samples by high-throughput se-quencing generated reads 32 088 766,the total length of 98 nt,sequencing base 3.1 Gb,8 types of antibiotic re-sistance were found,containing 21 kinds of resistant genotypes,a total of 16 polymorphic loci gene had 69 nu-cleotides,including 8 genotypes of 17 loci the occurrence of non-synonymous mutations,total 24.6% SNP loci. Conclusion Analysis of the use of multi drug resistant pathogen resistant bacteria has the feasibility of high-throughput gene sequencing technology hybrid genome.
ABSTRACT
<p><b>OBJECTIVE</b>To study the Arbuscular mycorrhizal (AM) formation progress and infection characteristics between tissue culture plantlets of Pinellia ternata and Glomus mosseae.</p><p><b>METHOD</b>The tissue culture plantlets of P. ternata were inoculated with G. mosseae, the formation of AM were sampled and observed with microscopy by staining.</p><p><b>RESULT AND CONCLUSION</b>The hyphae of G. mosseae began to penetrate the root epidermis after 10 days of inoculation. Lots of intracellular hyphae formed in cortex cells at the 15th day. Arbuscules started to form and there were some hyphae on the root at the 20th day. At the 25th day, many arbuscules formed and most as Arum type. Some arbuscles started to disintegrate at the 30th day, and a few of vesicles occurred. Lots of spores formed after 35 days. At the 40th day, some vesicles began to decline. The hand section showed that the intercellular hyphae gradually formed in intercellular space, and the hyphae branched in cortex cells and occupied most cell lumen finally. It is expounded that P. ternata and G. mosseae could recognize each other quickly and form a symbiont system.</p>
Subject(s)
Cell Culture Techniques , Cells, Cultured , Glomeromycota , Physiology , Hyphae , Physiology , Mycorrhizae , Physiology , Pinellia , Cell Biology , MicrobiologyABSTRACT
<p><b>OBJECTIVE</b>To research the mechanism of dormancy and find out the breaking method for the seeds of Epimedium wushanense.</p><p><b>METHOD</b>The water permeability of seed coat was tested by weighing seeds. The germination inhibitor of the seeds were determined with biotic measurement. The development of embryos, germination rate and germination potential were determined after stratification.</p><p><b>RESULT</b>The water permeability of seed coat was 41.86% after 5 h. The extracts of seeds had strong inhibition effects to the length growth of cabbage seedlings. The growth and development of embryos under the cold stratification (5 degrees C) were better than that under the other conditions. The embryo rate extended from 15.39% to 86.21% after 90 d. Germination rate and germination potential after stratification under 5 degrees C were significantly higher than that under other temperatures.</p><p><b>CONCLUSION</b>The results showed that there was no obstacle of water permeability on the test of E. wushanense, after-ripening of embryogenesis and the germination inhibitor of the seed were the main reason for the seed dormancy. The cold stratification would be an effective way for breaking of the dormancy, which could significantly promote the seed embryogenesis and increase germination rate comparing to other methods.</p>